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KMID : 0390119940340010159
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Journal of Pusan Medical College
1994 Volume.34 No. 1 p.159 ~ p.172
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Histopathological and Ultrastructural Studies on the Intrahepatic Bile Ducts in Rabbits Experimentally Infested with Clonorchis Sinensis
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Abstract
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It is well known that heavy infestation of Clonorchis sinesis(CS) n human might induce the cholangiocarcinoma, and there were several reports concerning about the cholangiocarcinoma animal models of cats and dogs induced by CS infestation. The
author
studied the morphological changes of the intrahepatic bile ducts after heavy infestation of CS in experimentally infested rabbits.
The adult rabbits were infested by eggs of CS and sacrified after 2 years of infestation. The pathological changes of bile ducts were studied with light microscopically, histochemically, and electron microscopically
@ES The results were summarized as follows:
@EN 1. Papillary proliferation of bile duct epithelium, adenomatous hyperplasia in the bile duct wall or extraductal areas, periductal fibrosis, goblet cell metaplasia, and mucinous metaplasia were identified in the intrahepatic bile ducts of the
rabbits infested by CS.
2. Mucin production was identified in the goblet cells and mucous glands cells of ductal epithelium infested by CS. And mucin histochemistry revealed thet the mucin of the goblet cell was mostly sulphated mucin and that of mucous gland was
mostly
class
III mucin.
3. Electron microscopic examination revealed that proliferated ductal cells were arranged in sheets or tubular pattern and invested with basal lamina, and interdigitation was well developed between the cells in most areas. Smoe areas of the
proliferated ductal cells had pleomorphic nuclei with irregular chromatin clumping and cytoplasms showed increased glycogen granules, decreased mitochondrial cristae, and proliferated and enlarged smooth endoplasmic reticulum. Cytoplasmic
protrusion
with edematous appearance into the biliary canaliculi and decreased microvilli with morphologic distorsion also noted in some areas.
In conclusion, evidence of precancerous changes in intrahepatic biliary system such as bile ductal epithelial proliferation, metaplastic changes o ductal cells, and ultrastructural changes of dysplasia or dedifferentiation was identified in the
rabbit
with CS infestation, but definite evidence of anaplastic changes identified in the human, cat, and dog was not identified in the rabbits used in this experiment. Therefore for the study of evidence of anaplastic changes in CS-infested rabbits, it
is
postulated that it should be needed for longer period of infestation.
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KEYWORD
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